Neurohistology
Links
Contact
Phone: 301-378-0505

Email: mbutt@toxpath.net



Standard hematoxylin and eosin (H&E) staining can reveal many tissue changes, but a thorough examination of the nervous system often requires a set of specialized processing and/or staining procedures, each designed to reveal more detail about a specific component of the brain, spin cord, ganglia or nerves.

Tox Path Specialists will help you design a study that incorporates the necessary processing and staining to make sure you obtain your study objectives avoid the expense of having to repeat studies. TPS has been involved in over 500 studies involving the nervous system, the majority of them GLP compliant, and we are familiar with the scientific and regulatory requirements pertinent to a wide variety of therapies and devices.

Explore the links on this page to learn the neurohistology capabilities of TPS.
Processing Techniques
Paraffin histology
  • Full tissue sets
  • Routine (5 micron) and thicker sections
  • Regular size and oversize (2x3) sections for full coronal/transverse brain sections of large species

Resin embedding (plastics)
  • Resin for nerve cross sections, brain/spinal cord and medical devices
  • Osmium post-fixation for myelin
  • Ultra-thin sections

Frozen sections
  • ​Gelatin embedded brain, spinal cord and ganglia sections
  • Thick sections suitable for immunohistochemistry and stereology
  • Sliding/freezing microtome for ultimate versatility

Specialized trimming and dissection
  • Trimming for particular brain regions, including substantia nigra, basal forebrain, striatum and many other specific brain regions
  • Dissection of dorsal root ganglia/spinal nerve roots
  • Dissection of sympathetic ganglia
  • See our Necropsy Support page to see what is possible at your laboratory
Staining
H&E (Standard)
GFAP (IHC)
Standard and histochemical stains
  • See what stains can be used to identify aspects of the nervous system that the standard H&E stain might fail to reveal
  • Stains include (but are not limited to) H&E, toluidine blue (TB), Fluoro Jade B (FJB) and more
  • Processing techniques include paraffin, resin embedding and frozen sections

Immunohistochemical stains​
  • Glial cells, nerve fibers, neuron sub-populations, and many other strutures/materials, including biologic test articles, can often be revealed with the proper immunohistochemical staining techniques
  • Stains include (but are not limited to) GFAP, NeuN, CGRP, tyrosine hydroxylase (TH) and more
  • Processing techniques include paraffin and frozen sections